Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4349 | 5' | -48.3 | NC_001720.1 | + | 18775 | 0.66 | 0.951125 |
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Target: 5'- -cUUCAGcaggucaauAGCAuCUCCgGGGugGUUCc -3' miRNA: 3'- gaAAGUCu--------UCGU-GAGGgUUCugCAAG- -5' |
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| 4349 | 5' | -48.3 | NC_001720.1 | + | 26709 | 0.66 | 0.940998 |
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Target: 5'- gCUUUCGGc-GCGCUCCCuuAGGCa--- -3' miRNA: 3'- -GAAAGUCuuCGUGAGGGu-UCUGcaag -5' |
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| 4349 | 5' | -48.3 | NC_001720.1 | + | 17215 | 0.67 | 0.923428 |
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Target: 5'- --cUCGGAgccGGCGgUUCCGAGGCGgaCg -3' miRNA: 3'- gaaAGUCU---UCGUgAGGGUUCUGCaaG- -5' |
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| 4349 | 5' | -48.3 | NC_001720.1 | + | 19446 | 0.67 | 0.916931 |
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Target: 5'- -cUUCAGAAGCACUgCCGu--CGUc- -3' miRNA: 3'- gaAAGUCUUCGUGAgGGUucuGCAag -5' |
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| 4349 | 5' | -48.3 | NC_001720.1 | + | 29799 | 0.7 | 0.815573 |
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Target: 5'- --gUCAGAAGCGC-CCCGAuGCGa-- -3' miRNA: 3'- gaaAGUCUUCGUGaGGGUUcUGCaag -5' |
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| 4349 | 5' | -48.3 | NC_001720.1 | + | 12839 | 1.11 | 0.002779 |
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Target: 5'- cCUUUCAGAAGCACUCCCAAGACGUUCu -3' miRNA: 3'- -GAAAGUCUUCGUGAGGGUUCUGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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