Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4359 | 3' | -55 | NC_001720.1 | + | 29040 | 0.68 | 0.552901 |
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Target: 5'- gUCUGACGG--CUGACgCGGACGGaauCUCc -3' miRNA: 3'- gAGACUGCUugGACUG-GUCUGCC---GAG- -5' |
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| 4359 | 3' | -55 | NC_001720.1 | + | 17722 | 0.68 | 0.552901 |
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Target: 5'- aUCUGGCGAAUCaGGCUcaAGAUuucggGGCUCu -3' miRNA: 3'- gAGACUGCUUGGaCUGG--UCUG-----CCGAG- -5' |
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| 4359 | 3' | -55 | NC_001720.1 | + | 40951 | 0.68 | 0.551803 |
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Target: 5'- gUCUcGCGAACCUccCCAGgguguucGCGGCUCc -3' miRNA: 3'- gAGAcUGCUUGGAcuGGUC-------UGCCGAG- -5' |
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| 4359 | 3' | -55 | NC_001720.1 | + | 9437 | 0.7 | 0.437501 |
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Target: 5'- uUCUGGC-AACCUcGcCCGGAUGGCUg -3' miRNA: 3'- gAGACUGcUUGGA-CuGGUCUGCCGAg -5' |
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| 4359 | 3' | -55 | NC_001720.1 | + | 9682 | 0.7 | 0.399041 |
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Target: 5'- gUCUGACGAugCUGcagcaGCguGACGGUg- -3' miRNA: 3'- gAGACUGCUugGAC-----UGguCUGCCGag -5' |
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| 4359 | 3' | -55 | NC_001720.1 | + | 17110 | 0.98 | 0.005056 |
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Target: 5'- gCUCUGACGAACCUaGCCAGACGGCUCc -3' miRNA: 3'- -GAGACUGCUUGGAcUGGUCUGCCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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