miRNA: 3'- -AUCUGCa--CGGCuGGUGCGACCUUUAc -5'
miRNA: 3'- auCUGC----ACGGCugGUGCgACCUUUAC- -5'
miRNA: 3'- auCUGCA-CGGCuGGUGCGACCUUuac -5'
miRNA: 3'- auCUGCA--CGGCUGGUGCGaCCUUUac -5'
miRNA: 3'- -AUCUGCACGGCUGGUGCGACCUUUAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4361 | 5' | -53.8 | NC_001720.1 | + | 6267 | 0.66 | 0.723499 |
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Target: 5'- aUGGACGgcaGCUGuCCGUGUUGGAGGUa -3' miRNA: 3'- -AUCUGCa--CGGCuGGUGCGACCUUUAc -5' |
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| 4361 | 5' | -53.8 | NC_001720.1 | + | 22413 | 0.69 | 0.531751 |
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Target: 5'- -uGGCGcaacUGCCGugCGCG-UGGGGAUGa -3' miRNA: 3'- auCUGC----ACGGCugGUGCgACCUUUAC- -5' |
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| 4361 | 5' | -53.8 | NC_001720.1 | + | 26607 | 0.71 | 0.397691 |
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Target: 5'- -cGGCGUaGUCG-CCGCGCUGGAGc-- -3' miRNA: 3'- auCUGCA-CGGCuGGUGCGACCUUuac -5' |
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| 4361 | 5' | -53.8 | NC_001720.1 | + | 35033 | 0.73 | 0.326713 |
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Target: 5'- -uGACGUagGCUGACCACGC-GGAAGg- -3' miRNA: 3'- auCUGCA--CGGCUGGUGCGaCCUUUac -5' |
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| 4361 | 5' | -53.8 | NC_001720.1 | + | 18069 | 1.06 | 0.001534 |
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Target: 5'- gUAGACGUGCCGACCACGCUGGAAAUGc -3' miRNA: 3'- -AUCUGCACGGCUGGUGCGACCUUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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