Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4364 | 3' | -57.2 | NC_001720.1 | + | 37689 | 0.66 | 0.547318 |
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Target: 5'- cGCgUUCGCGGuucCUGaUUGGGUgGCCAg -3' miRNA: 3'- cUGgAAGCGCCu--GAUgAACCCG-CGGU- -5' |
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| 4364 | 3' | -57.2 | NC_001720.1 | + | 27357 | 0.66 | 0.547318 |
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Target: 5'- cGGCCgUUGCGGAUUGCgUGGcauuuucuaaGCGCUg -3' miRNA: 3'- -CUGGaAGCGCCUGAUGaACC----------CGCGGu -5' |
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| 4364 | 3' | -57.2 | NC_001720.1 | + | 32409 | 0.66 | 0.525775 |
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Target: 5'- aGCCUUCGCGGAgCUagacacccagcaACUggugcGGGaUGCCGa -3' miRNA: 3'- cUGGAAGCGCCU-GA------------UGAa----CCC-GCGGU- -5' |
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| 4364 | 3' | -57.2 | NC_001720.1 | + | 11675 | 0.7 | 0.288234 |
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Target: 5'- --aCUUCGUGG-CUAUgUGGGCGUCAc -3' miRNA: 3'- cugGAAGCGCCuGAUGaACCCGCGGU- -5' |
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| 4364 | 3' | -57.2 | NC_001720.1 | + | 24970 | 0.71 | 0.253467 |
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Target: 5'- uGGCCaUCGUGGACccgACa-GGGCGCCu -3' miRNA: 3'- -CUGGaAGCGCCUGa--UGaaCCCGCGGu -5' |
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| 4364 | 3' | -57.2 | NC_001720.1 | + | 20100 | 1.08 | 0.000482 |
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Target: 5'- aGACCUUCGCGGACUACUUGGGCGCCAa -3' miRNA: 3'- -CUGGAAGCGCCUGAUGAACCCGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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