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miRNA: 3'- ccUGAaauaCAUAUACCCUCuCGACU- -5'
miRNA: 3'- -CCUGaaaua-CAUAUa-CCCUCUCGacu -5'
miRNA: 3'- -CCUGaaaUACAua---UACCCUCUCGACU- -5'
miRNA: 3'- -CCUGAAAUACAUAUACCCUCUCGACU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 4366 | 3' | -45.4 | NC_001720.1 | + | 9640 | 0.66 | 0.994638 |
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Target: 5'- -aACUgac-GUAUGUGGGGGuGUUGGg -3' miRNA: 3'- ccUGAaauaCAUAUACCCUCuCGACU- -5' |
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| 4366 | 3' | -45.4 | NC_001720.1 | + | 26341 | 0.68 | 0.973925 |
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Target: 5'- cGGGCccggagGUGUAcgGGGAGAGCg-- -3' miRNA: 3'- -CCUGaaaua-CAUAUa-CCCUCUCGacu -5' |
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| 4366 | 3' | -45.4 | NC_001720.1 | + | 4679 | 0.81 | 0.441585 |
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Target: 5'- uGGGCag-AUGUucgggAUGGGGGAGCUGAu -3' miRNA: 3'- -CCUGaaaUACAua---UACCCUCUCGACU- -5' |
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| 4366 | 3' | -45.4 | NC_001720.1 | + | 20871 | 1.14 | 0.004276 |
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Target: 5'- cGGACUUUAUGUAUAUGGGAGAGCUGAc -3' miRNA: 3'- -CCUGAAAUACAUAUACCCUCUCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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