Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4369 | 5' | -59.3 | NC_001720.1 | + | 12451 | 0.66 | 0.439732 |
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Target: 5'- cGGGACGCCGCGcGGCgagccACGGUgc--CCGa -3' miRNA: 3'- -UUCUGCGGCGC-CUG-----UGCCGacuaGGC- -5' |
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| 4369 | 5' | -59.3 | NC_001720.1 | + | 38966 | 0.66 | 0.430152 |
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Target: 5'- -cGugGCUucauuaauaGCGGACAUGcGCaGAUCCa -3' miRNA: 3'- uuCugCGG---------CGCCUGUGC-CGaCUAGGc -5' |
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| 4369 | 5' | -59.3 | NC_001720.1 | + | 24328 | 0.66 | 0.402167 |
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Target: 5'- cGGGACGCCcucGUGGcCcCGGCcGAUCCu -3' miRNA: 3'- -UUCUGCGG---CGCCuGuGCCGaCUAGGc -5' |
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| 4369 | 5' | -59.3 | NC_001720.1 | + | 32085 | 0.71 | 0.211536 |
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Target: 5'- -cGugGCCGCGGACAUGGg----CCGg -3' miRNA: 3'- uuCugCGGCGCCUGUGCCgacuaGGC- -5' |
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| 4369 | 5' | -59.3 | NC_001720.1 | + | 25790 | 0.74 | 0.126437 |
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Target: 5'- -cGGCGCCGCGGGUGCGGCgGGcggcucUCCGg -3' miRNA: 3'- uuCUGCGGCGCCUGUGCCGaCU------AGGC- -5' |
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| 4369 | 5' | -59.3 | NC_001720.1 | + | 24415 | 1.05 | 0.000555 |
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Target: 5'- aAAGACGCCGCGGACACGGCUGAUCCGc -3' miRNA: 3'- -UUCUGCGGCGCCUGUGCCGACUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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