Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4370 | 3' | -56 | NC_001720.1 | + | 18600 | 0.66 | 0.627563 |
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Target: 5'- aCGAaGCCGUGGuggaagguauGGUGGAGGaCCu -3' miRNA: 3'- aGUUcCGGCACCuuu-------UCGCCUCCcGG- -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 32770 | 0.66 | 0.596418 |
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Target: 5'- aCAGGaugaugcuGCUGUaGGAcAGGGCgaccaGGAGGGCCa -3' miRNA: 3'- aGUUC--------CGGCA-CCU-UUUCG-----CCUCCCGG- -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 16794 | 0.67 | 0.552392 |
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Target: 5'- aCGuGGCgGUcGGGAcGGCGGcGGGCg -3' miRNA: 3'- aGUuCCGgCA-CCUUuUCGCCuCCCGg -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 28239 | 0.67 | 0.552392 |
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Target: 5'- -gGGGGCgGaGGAggagGAGGCGG-GGGCg -3' miRNA: 3'- agUUCCGgCaCCU----UUUCGCCuCCCGg -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 12416 | 0.68 | 0.492584 |
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Target: 5'- cCGGGGCCGgugUGGAcgaaguuagaaugagGGAGCGGGacGCCg -3' miRNA: 3'- aGUUCCGGC---ACCU---------------UUUCGCCUccCGG- -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 10065 | 0.68 | 0.467864 |
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Target: 5'- aUggGGaauacaGUGGGuugGAAGCGGAaGGGCUg -3' miRNA: 3'- aGuuCCgg----CACCU---UUUCGCCU-CCCGG- -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 15456 | 0.69 | 0.447761 |
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Target: 5'- aCAauAGGUCGUGuuGGGGCGGAGaGCUa -3' miRNA: 3'- aGU--UCCGGCACcuUUUCGCCUCcCGG- -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 25757 | 0.69 | 0.43789 |
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Target: 5'- aCGA-GUgGUGGGcacGAGCGGcGGGGCCg -3' miRNA: 3'- aGUUcCGgCACCUu--UUCGCC-UCCCGG- -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 14063 | 0.69 | 0.418531 |
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Target: 5'- -gGAGGCUaucagagccgauGUGGAc--GCGGAGGGUg -3' miRNA: 3'- agUUCCGG------------CACCUuuuCGCCUCCCGg -5' |
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| 4370 | 3' | -56 | NC_001720.1 | + | 25014 | 1.13 | 0.000334 |
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Target: 5'- cUCAAGGCCGUGGAAAAGCGGAGGGCCg -3' miRNA: 3'- -AGUUCCGGCACCUUUUCGCCUCCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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