Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4370 | 5' | -59.1 | NC_001720.1 | + | 5210 | 0.66 | 0.437142 |
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Target: 5'- -aGGCGGUGCGCauaaaccacuccccuUGgACUuuGACCCa -3' miRNA: 3'- uaCCGCCAUGCG---------------AUgUGGcgCUGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 26852 | 0.68 | 0.33554 |
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Target: 5'- -aGGCuGUGCGuCUGCGCCaaGAUCCg -3' miRNA: 3'- uaCCGcCAUGC-GAUGUGGcgCUGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 25775 | 0.68 | 0.311066 |
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Target: 5'- -cGGCGGggccgGCGCcgGCGCCGCGggugcggcgggcgGCUCUc -3' miRNA: 3'- uaCCGCCa----UGCGa-UGUGGCGC-------------UGGGA- -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 10567 | 0.68 | 0.302713 |
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Target: 5'- cAUGGUGGaaacuauuucuCGCagcaGCGCCGUGACCCa -3' miRNA: 3'- -UACCGCCau---------GCGa---UGUGGCGCUGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 31691 | 0.69 | 0.296748 |
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Target: 5'- -cGGUGGUGCauaccugucccGCUGC-CCGCGcCCCc -3' miRNA: 3'- uaCCGCCAUG-----------CGAUGuGGCGCuGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 26446 | 0.69 | 0.26153 |
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Target: 5'- --aGCGGUGCGCuUGCGCCGCcuUCCg -3' miRNA: 3'- uacCGCCAUGCG-AUGUGGCGcuGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 17289 | 0.7 | 0.248424 |
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Target: 5'- -cGGCGGaaggcgGCGCaagcGCACCGCuACCCg -3' miRNA: 3'- uaCCGCCa-----UGCGa---UGUGGCGcUGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 25568 | 0.71 | 0.190877 |
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Target: 5'- aGUGGCGGU-CGCUACuCCG-GuACCCg -3' miRNA: 3'- -UACCGCCAuGCGAUGuGGCgC-UGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 28861 | 0.73 | 0.149432 |
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Target: 5'- cUGGCGGcGgGCU-CACCGCuGACCCc -3' miRNA: 3'- uACCGCCaUgCGAuGUGGCG-CUGGGa -5' |
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| 4370 | 5' | -59.1 | NC_001720.1 | + | 25049 | 1.05 | 0.000605 |
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Target: 5'- uAUGGCGGUACGCUACACCGCGACCCUc -3' miRNA: 3'- -UACCGCCAUGCGAUGUGGCGCUGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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