Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
4372 | 5' | -56.5 | NC_001720.1 | + | 12406 | 0.66 | 0.565203 |
Target: 5'- gGGCUgGCgGCCgGGGCcggUGUGGACgAAg -3' miRNA: 3'- -UCGAgUG-CGGaCUCG---ACACCUGgUUg -5' |
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4372 | 5' | -56.5 | NC_001720.1 | + | 37007 | 0.66 | 0.554138 |
Target: 5'- cAGCUCACGCgaGuGCUgcguccGUGGAagGACc -3' miRNA: 3'- -UCGAGUGCGgaCuCGA------CACCUggUUG- -5' |
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4372 | 5' | -56.5 | NC_001720.1 | + | 8845 | 0.67 | 0.489371 |
Target: 5'- gGGCa-AUGCCgcgGAGUUGUGGACUuauGACc -3' miRNA: 3'- -UCGagUGCGGa--CUCGACACCUGG---UUG- -5' |
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4372 | 5' | -56.5 | NC_001720.1 | + | 25875 | 1.1 | 0.000381 |
Target: 5'- aAGCUCACGCCUGAGCUGUGGACCAACg -3' miRNA: 3'- -UCGAGUGCGGACUCGACACCUGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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