Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4373 | 5' | -50.7 | NC_001720.1 | + | 11171 | 0.67 | 0.821475 |
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Target: 5'- cGGCUGCAGAGGGuGauGGCGcc-UCCu -3' miRNA: 3'- aCUGGCGUUUUUC-CgcCCGCuuuAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 10865 | 0.66 | 0.890814 |
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Target: 5'- cGACgCGCGAGAGucGCuGGUGAAcGUCCg -3' miRNA: 3'- aCUG-GCGUUUUUc-CGcCCGCUU-UAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 9925 | 0.68 | 0.801748 |
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Target: 5'- aUGACgGUGAccuGGGCGGGCuuaAAGUCg -3' miRNA: 3'- -ACUGgCGUUuu-UCCGCCCGc--UUUAGg -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 4944 | 0.66 | 0.875068 |
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Target: 5'- uUGA-UGCu--GGGGUGGGCGcgGUCUa -3' miRNA: 3'- -ACUgGCGuuuUUCCGCCCGCuuUAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 1233 | 0.68 | 0.801748 |
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Target: 5'- aGACCGCG---GGGCgucGGGUuucGGGUCCa -3' miRNA: 3'- aCUGGCGUuuuUCCG---CCCGc--UUUAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 1042 | 0.67 | 0.825318 |
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Target: 5'- uUGGCCGCcAAAuuuuucauugacgucGGaGCGGGUGuuaucGAUCCc -3' miRNA: 3'- -ACUGGCGuUUU---------------UC-CGCCCGCu----UUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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