Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4375 | 5' | -53.7 | NC_001720.1 | + | 33469 | 0.66 | 0.797323 |
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Target: 5'- uCGAAgCGGCGg-CGCGACGagGUCAg -3' miRNA: 3'- cGCUUgGCUGCaaGCGCUGUggCAGU- -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 10512 | 0.66 | 0.791429 |
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Target: 5'- uGCGGucuCCGguuguucucaugaauGCGUgUCGCGugACCGUg- -3' miRNA: 3'- -CGCUu--GGC---------------UGCA-AGCGCugUGGCAgu -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 13056 | 0.66 | 0.787466 |
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Target: 5'- cGUGAGCCuGACGUUCaacaaGCCGUCc -3' miRNA: 3'- -CGCUUGG-CUGCAAGcgcugUGGCAGu -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 31800 | 0.66 | 0.756951 |
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Target: 5'- cGCGAucGCCGaACG-UCGCcGCGCCGcCc -3' miRNA: 3'- -CGCU--UGGC-UGCaAGCGcUGUGGCaGu -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 27828 | 0.67 | 0.746505 |
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Target: 5'- -aGGGCUcGCGcUUCGCGAUACCGagCAg -3' miRNA: 3'- cgCUUGGcUGC-AAGCGCUGUGGCa-GU- -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 11944 | 0.67 | 0.703653 |
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Target: 5'- gGCGAcguUCGGCGaUCGCGGCGCgagGUCu -3' miRNA: 3'- -CGCUu--GGCUGCaAGCGCUGUGg--CAGu -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 30742 | 0.67 | 0.703653 |
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Target: 5'- cGCGAucggggucaagACCGACGgaaGCauUACCGUCAa -3' miRNA: 3'- -CGCU-----------UGGCUGCaagCGcuGUGGCAGU- -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 17781 | 0.67 | 0.692733 |
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Target: 5'- uGCGAACCGcgauGCGUUCuCGggauaguggaACACCGUgAa -3' miRNA: 3'- -CGCUUGGC----UGCAAGcGC----------UGUGGCAgU- -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 17470 | 0.79 | 0.175258 |
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Target: 5'- uGCGGGCCGACuUUCGCGAUAggaaacugcCCGUCu -3' miRNA: 3'- -CGCUUGGCUGcAAGCGCUGU---------GGCAGu -5' |
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| 4375 | 5' | -53.7 | NC_001720.1 | + | 28150 | 1.11 | 0.000946 |
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Target: 5'- cGCGAACCGACGUUCGCGACACCGUCAg -3' miRNA: 3'- -CGCUUGGCUGCAAGCGCUGUGGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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