Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4376 | 3' | -58.6 | NC_001720.1 | + | 32859 | 0.66 | 0.454584 |
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Target: 5'- aCUGCaagACGa-GCuCAGCGGGGCCGc -3' miRNA: 3'- -GGCGaaaUGCggCG-GUCGCCCUGGUa -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 12029 | 0.67 | 0.425499 |
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Target: 5'- aCCGUaacggggGCGCgGgCAGCGGGACa-- -3' miRNA: 3'- -GGCGaaa----UGCGgCgGUCGCCCUGgua -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 17311 | 0.67 | 0.397551 |
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Target: 5'- aCCGCUaccCGCCGCCgaucucugaAGgGGGGCUu- -3' miRNA: 3'- -GGCGAaauGCGGCGG---------UCgCCCUGGua -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 29301 | 0.68 | 0.362174 |
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Target: 5'- cCUGCcggAgGCCGCCAGuUGGGGuCCAg -3' miRNA: 3'- -GGCGaaaUgCGGCGGUC-GCCCU-GGUa -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 16551 | 0.69 | 0.321119 |
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Target: 5'- gCCGUaauucugUUGCG-CGCCGGCGGGaauGCCGg -3' miRNA: 3'- -GGCGa------AAUGCgGCGGUCGCCC---UGGUa -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 20730 | 0.72 | 0.202479 |
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Target: 5'- -gGCgagUUAC-CCGCCGGCGGGAaaCCGUu -3' miRNA: 3'- ggCGa--AAUGcGGCGGUCGCCCU--GGUA- -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 22099 | 0.76 | 0.102837 |
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Target: 5'- uCCGCccgACGCCGCCuGCGGGuugcagcaggugaacACCAUg -3' miRNA: 3'- -GGCGaaaUGCGGCGGuCGCCC---------------UGGUA- -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 29939 | 0.79 | 0.062499 |
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Target: 5'- gCGCUgcaggugGCGUgGUCAGCGGGACCAUu -3' miRNA: 3'- gGCGAaa-----UGCGgCGGUCGCCCUGGUA- -5' |
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| 4376 | 3' | -58.6 | NC_001720.1 | + | 28298 | 1.07 | 0.000496 |
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Target: 5'- cCCGCUUUACGCCGCCAGCGGGACCAUc -3' miRNA: 3'- -GGCGAAAUGCGGCGGUCGCCCUGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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