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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4377 | 5' | -62.7 | NC_001720.1 | + | 26236 | 0.66 | 0.303817 |
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Target: 5'- aCGGC--UugCCCCGGAcuaccaaGACGGGCa- -3' miRNA: 3'- aGUCGgaGugGGGGCCU-------CUGCCCGag -5' |
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| 4377 | 5' | -62.7 | NC_001720.1 | + | 21249 | 0.66 | 0.29728 |
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Target: 5'- cCGGCUcCcggGCCUCCGGAGGuaugcacuggauCGGGUUCg -3' miRNA: 3'- aGUCGGaG---UGGGGGCCUCU------------GCCCGAG- -5' |
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| 4377 | 5' | -62.7 | NC_001720.1 | + | 20222 | 0.67 | 0.262946 |
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Target: 5'- --cGCCUCAaggCCUCGGAGACgccccagcugGGcGCUCa -3' miRNA: 3'- aguCGGAGUg--GGGGCCUCUG----------CC-CGAG- -5' |
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| 4377 | 5' | -62.7 | NC_001720.1 | + | 28745 | 0.69 | 0.174417 |
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Target: 5'- gUAGCUUgCGCCCCCGGcgcGACGcGGC-Cg -3' miRNA: 3'- aGUCGGA-GUGGGGGCCu--CUGC-CCGaG- -5' |
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| 4377 | 5' | -62.7 | NC_001720.1 | + | 28422 | 1.09 | 0.000127 |
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Target: 5'- gUCAGCCUCACCCCCGGAGACGGGCUCg -3' miRNA: 3'- -AGUCGGAGUGGGGGCCUCUGCCCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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