Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4378 | 5' | -53.1 | NC_001720.1 | + | 33531 | 0.66 | 0.804231 |
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Target: 5'- -aCGGAcagCGugCUGGGCCCCGAUu- -3' miRNA: 3'- gaGCUUca-GUugGAUCUGGGGCUGcc -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 20890 | 0.67 | 0.764073 |
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Target: 5'- --gGGAGcCAGCgUGcGCCCCGACGa -3' miRNA: 3'- gagCUUCaGUUGgAUcUGGGGCUGCc -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 33039 | 0.67 | 0.721683 |
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Target: 5'- uUCGAcGUUAGCgUAGGacaaCCGACGGu -3' miRNA: 3'- gAGCUuCAGUUGgAUCUgg--GGCUGCC- -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 28415 | 0.69 | 0.655582 |
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Target: 5'- gCUgGgcGUCAGCCUc-ACCCCcggaGACGGg -3' miRNA: 3'- -GAgCuuCAGUUGGAucUGGGG----CUGCC- -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 23480 | 0.7 | 0.57757 |
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Target: 5'- uCUCGggGUCuuUC--GGCCCCGuCGGu -3' miRNA: 3'- -GAGCuuCAGuuGGauCUGGGGCuGCC- -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 20185 | 0.7 | 0.555559 |
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Target: 5'- cCUCGAAG--AGCCcGGGCCgUCGACGGu -3' miRNA: 3'- -GAGCUUCagUUGGaUCUGG-GGCUGCC- -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 28867 | 0.7 | 0.544644 |
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Target: 5'- -gCGGGcUC-ACCgcuGACCCCGACGGu -3' miRNA: 3'- gaGCUUcAGuUGGau-CUGGGGCUGCC- -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 28619 | 0.73 | 0.402003 |
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Target: 5'- --aGGAGUCAAgCUcGAUCCCGACGa -3' miRNA: 3'- gagCUUCAGUUgGAuCUGGGGCUGCc -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 29275 | 0.76 | 0.278199 |
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Target: 5'- gCUCGAGGUCAACacGGACCCgaGCGGa -3' miRNA: 3'- -GAGCUUCAGUUGgaUCUGGGgcUGCC- -5' |
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| 4378 | 5' | -53.1 | NC_001720.1 | + | 28517 | 1.11 | 0.001079 |
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Target: 5'- gCUCGAAGUCAACCUAGACCCCGACGGa -3' miRNA: 3'- -GAGCUUCAGUUGGAUCUGGGGCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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