Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4379 | 3' | -53.5 | NC_001720.1 | + | 23152 | 0.66 | 0.762708 |
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Target: 5'- aCAGGCUUGGUgCUG-CGAAaGCCAUg- -3' miRNA: 3'- -GUUCGAGCUAgGGCuGCUU-CGGUAgc -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 7623 | 0.66 | 0.762708 |
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Target: 5'- uCGGGCUCu-UCCUGuuCGggGUCGUCu -3' miRNA: 3'- -GUUCGAGcuAGGGCu-GCuuCGGUAGc -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 22627 | 0.66 | 0.752174 |
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Target: 5'- uGGGuCUCGGugguUCCCGACaucccAGCCGUCu -3' miRNA: 3'- gUUC-GAGCU----AGGGCUGcu---UCGGUAGc -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 28306 | 0.67 | 0.697829 |
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Target: 5'- uCGAGCUgaaUGGUCuCUGugGcGGCCGUUGa -3' miRNA: 3'- -GUUCGA---GCUAG-GGCugCuUCGGUAGC- -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 2294 | 0.67 | 0.68671 |
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Target: 5'- aCAGcGCU-GAUCCgGugGGcgGGCCAUCa -3' miRNA: 3'- -GUU-CGAgCUAGGgCugCU--UCGGUAGc -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 14270 | 0.68 | 0.653072 |
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Target: 5'- aCAGGCUCuuaaggacggGGUCCCGGCGGAcauuuCUAUCa -3' miRNA: 3'- -GUUCGAG----------CUAGGGCUGCUUc----GGUAGc -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 12374 | 0.74 | 0.311527 |
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Target: 5'- --cGCUUGAUCCCGAUGAAGgaCCgGUCGc -3' miRNA: 3'- guuCGAGCUAGGGCUGCUUC--GG-UAGC- -5' |
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| 4379 | 3' | -53.5 | NC_001720.1 | + | 28624 | 1.09 | 0.001207 |
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Target: 5'- uCAAGCUCGAUCCCGACGAAGCCAUCGa -3' miRNA: 3'- -GUUCGAGCUAGGGCUGCUUCGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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