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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4381 | 3' | -59.9 | NC_001720.1 | + | 25410 | 0.66 | 0.402167 |
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Target: 5'- aCUCUCGGGUg--CCAgGGCC-CGCg- -3' miRNA: 3'- -GAGAGCCCGagaGGUaUCGGcGCGgu -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 26590 | 0.66 | 0.366705 |
|
Target: 5'- gUCggCGGGC-CUCCAagcggcguagUcGCCGCGCUg -3' miRNA: 3'- gAGa-GCCCGaGAGGU----------AuCGGCGCGGu -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 28834 | 0.71 | 0.190263 |
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Target: 5'- gUCgUCGGGCggaggC-CCAcGGCCGUGCCGg -3' miRNA: 3'- gAG-AGCCCGa----GaGGUaUCGGCGCGGU- -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 29414 | 1.07 | 0.000316 |
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Target: 5'- uCUCUCGGGCUCUCCAUAGCCGCGCCAc -3' miRNA: 3'- -GAGAGCCCGAGAGGUAUCGGCGCGGU- -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 31007 | 0.69 | 0.260228 |
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Target: 5'- aUCUCGGGC-CUUaacagAGCCGUGCgGa -3' miRNA: 3'- gAGAGCCCGaGAGgua--UCGGCGCGgU- -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 31277 | 0.69 | 0.253674 |
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Target: 5'- aUCUCGGGCa--CCGUGgcucGCCGCGCg- -3' miRNA: 3'- gAGAGCCCGagaGGUAU----CGGCGCGgu -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 34801 | 0.69 | 0.260228 |
|
Target: 5'- gCUCaccagCGGGgccaaaCUCUCCAaAGCCGCGgCAg -3' miRNA: 3'- -GAGa----GCCC------GAGAGGUaUCGGCGCgGU- -5' |
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| 4381 | 3' | -59.9 | NC_001720.1 | + | 36427 | 0.66 | 0.372753 |
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Target: 5'- aUCgCGGGCucuuauacacuuacUCUCCGcccauucgaaAGCCGCGCCu -3' miRNA: 3'- gAGaGCCCG--------------AGAGGUa---------UCGGCGCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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