Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4382 | 3' | -55.5 | NC_001720.1 | + | 28965 | 0.66 | 0.634414 |
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Target: 5'- gAACGGCuACGCaugugggaAUCGGGGggCAAcGGCg -3' miRNA: 3'- -UUGCCGuUGCGc-------UAGCCCCa-GUU-CUG- -5' |
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| 4382 | 3' | -55.5 | NC_001720.1 | + | 37499 | 0.66 | 0.589456 |
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Target: 5'- cAugGGCAAgcaGCccaucAUCGGGGUCAucuacGGACc -3' miRNA: 3'- -UugCCGUUg--CGc----UAGCCCCAGU-----UCUG- -5' |
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| 4382 | 3' | -55.5 | NC_001720.1 | + | 14849 | 0.67 | 0.556096 |
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Target: 5'- cGACGGUGGcCGUGAccgagauaccgUCGGGGUCA--GCg -3' miRNA: 3'- -UUGCCGUU-GCGCU-----------AGCCCCAGUucUG- -5' |
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| 4382 | 3' | -55.5 | NC_001720.1 | + | 33472 | 0.7 | 0.382834 |
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Target: 5'- aAGCGGCGGCGCGA-CGaGGUCAGc-- -3' miRNA: 3'- -UUGCCGUUGCGCUaGCcCCAGUUcug -5' |
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| 4382 | 3' | -55.5 | NC_001720.1 | + | 19242 | 0.75 | 0.180137 |
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Target: 5'- cGGCgGGCAcGCgGCGGUCGGGGUUaAAGACg -3' miRNA: 3'- -UUG-CCGU-UG-CGCUAGCCCCAG-UUCUG- -5' |
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| 4382 | 3' | -55.5 | NC_001720.1 | + | 30733 | 1.06 | 0.001057 |
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Target: 5'- uAACGGCAACGCGAUCGGGGUCAAGACc -3' miRNA: 3'- -UUGCCGUUGCGCUAGCCCCAGUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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