Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4383 | 3' | -51.9 | NC_001720.1 | + | 4568 | 0.66 | 0.816813 |
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Target: 5'- gGCcGCCGCCc-CGGUuUCGAUUUUGu -3' miRNA: 3'- gUGaUGGCGGuuGCCGuAGCUAGAAU- -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 19705 | 0.66 | 0.796844 |
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Target: 5'- uGCUGCaaCGCCGagauccucuACGGCGUCGcUCUc- -3' miRNA: 3'- gUGAUG--GCGGU---------UGCCGUAGCuAGAau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 19454 | 0.66 | 0.796844 |
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Target: 5'- gCACUGCCGUCGucGCGGUgaguGUCGucGUCa-- -3' miRNA: 3'- -GUGAUGGCGGU--UGCCG----UAGC--UAGaau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 23547 | 0.66 | 0.796844 |
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Target: 5'- gAUUACCGUCGACGGCc-CGGgcUCUUc -3' miRNA: 3'- gUGAUGGCGGUUGCCGuaGCU--AGAAu -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 28556 | 0.67 | 0.765498 |
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Target: 5'- ---aACCGCCAcgGGgAUCGAUCUg- -3' miRNA: 3'- gugaUGGCGGUugCCgUAGCUAGAau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 28876 | 0.68 | 0.710404 |
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Target: 5'- cCGCUgACC-CCGACGGUAucUCGGUCa-- -3' miRNA: 3'- -GUGA-UGGcGGUUGCCGU--AGCUAGaau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 16715 | 0.69 | 0.638151 |
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Target: 5'- -gUUACCGCCGuagagcauccaugcGCGGgGUCGGUCg-- -3' miRNA: 3'- guGAUGGCGGU--------------UGCCgUAGCUAGaau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 22379 | 0.72 | 0.441557 |
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Target: 5'- gCGCUgACCGCCuugGCGGCGUCGGa---- -3' miRNA: 3'- -GUGA-UGGCGGu--UGCCGUAGCUagaau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 17404 | 0.76 | 0.289182 |
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Target: 5'- gGC-ACCGCCGugGGCAUCGccaAUCUg- -3' miRNA: 3'- gUGaUGGCGGUugCCGUAGC---UAGAau -5' |
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| 4383 | 3' | -51.9 | NC_001720.1 | + | 30828 | 1.07 | 0.001963 |
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Target: 5'- cCACUACCGCCAACGGCAUCGAUCUUAa -3' miRNA: 3'- -GUGAUGGCGGUUGCCGUAGCUAGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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