Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4383 | 5' | -50.8 | NC_001720.1 | + | 15341 | 0.66 | 0.894251 |
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Target: 5'- gCCCAGcuGGCCUUGcGU-GUUGAgGGUGa -3' miRNA: 3'- -GGGUU--UUGGGAC-CAgCAACUgCCAUc -5' |
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| 4383 | 5' | -50.8 | NC_001720.1 | + | 9702 | 0.66 | 0.87895 |
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Target: 5'- gCCCGAu-CCCUGGaCGgcG-UGGUAGu -3' miRNA: 3'- -GGGUUuuGGGACCaGCaaCuGCCAUC- -5' |
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| 4383 | 5' | -50.8 | NC_001720.1 | + | 28848 | 0.67 | 0.845134 |
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Target: 5'- gCCCAcgGCCgUgccGGUCGgUUGACGGc-- -3' miRNA: 3'- -GGGUuuUGGgA---CCAGC-AACUGCCauc -5' |
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| 4383 | 5' | -50.8 | NC_001720.1 | + | 14970 | 0.67 | 0.836056 |
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Target: 5'- gCCAGGucgauGCCCUGGUCGUccGCaGUAa -3' miRNA: 3'- gGGUUU-----UGGGACCAGCAacUGcCAUc -5' |
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| 4383 | 5' | -50.8 | NC_001720.1 | + | 4611 | 0.68 | 0.81722 |
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Target: 5'- uCCCAgaaaGAACCCgcaGcCGUUGGCGGg-- -3' miRNA: 3'- -GGGU----UUUGGGac-CaGCAACUGCCauc -5' |
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| 4383 | 5' | -50.8 | NC_001720.1 | + | 20184 | 0.71 | 0.644654 |
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Target: 5'- aCCUcgaAGAGCCCgGGcCGUcGACGGUAa -3' miRNA: 3'- -GGG---UUUUGGGaCCaGCAaCUGCCAUc -5' |
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| 4383 | 5' | -50.8 | NC_001720.1 | + | 30862 | 1.1 | 0.002091 |
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Target: 5'- uCCCAAAACCCUGGUCGUUGACGGUAGc -3' miRNA: 3'- -GGGUUUUGGGACCAGCAACUGCCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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