Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4387 | 3' | -54.8 | NC_001720.1 | + | 709 | 0.66 | 0.709 |
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Target: 5'- gUCGCaaCCUAucGGGGGCAGCauuaUGGUGAGu -3' miRNA: 3'- -GGCG--GGGUu-UUUCCGUUGg---GCCACUC- -5' |
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| 4387 | 3' | -54.8 | NC_001720.1 | + | 15235 | 0.67 | 0.642502 |
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Target: 5'- gCCGCUCCAGAGGGugauGCcGCCUacgGGUGGa -3' miRNA: 3'- -GGCGGGGUUUUUC----CGuUGGG---CCACUc -5' |
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| 4387 | 3' | -54.8 | NC_001720.1 | + | 18104 | 0.7 | 0.458562 |
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Target: 5'- gCCGCCCaagcgcaggcgCAAGAGGGCAcgaCCGGgacaaugGAGg -3' miRNA: 3'- -GGCGGG-----------GUUUUUCCGUug-GGCCa------CUC- -5' |
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| 4387 | 3' | -54.8 | NC_001720.1 | + | 32552 | 0.71 | 0.393758 |
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Target: 5'- cCCGCCCCGugaaccaggccagcgGGAaaucggugcagguAGGgGAUCCGGUGAa -3' miRNA: 3'- -GGCGGGGU---------------UUU-------------UCCgUUGGGCCACUc -5' |
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| 4387 | 3' | -54.8 | NC_001720.1 | + | 40197 | 1.11 | 0.000618 |
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Target: 5'- cCCGCCCCAAAAAGGCAACCCGGUGAGa -3' miRNA: 3'- -GGCGGGGUUUUUCCGUUGGGCCACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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