Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4388 | 3' | -58.3 | NC_001720.1 | + | 20610 | 0.66 | 0.469876 |
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Target: 5'- uGGacaCCACGCUguacgACCugGUGUCCa -3' miRNA: 3'- uUCg--GGUGCGGgua--UGGugCACAGGc -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 26343 | 0.67 | 0.420824 |
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Target: 5'- -uGCCCACGgCgGUGCCuGCGaUGcCCGg -3' miRNA: 3'- uuCGGGUGCgGgUAUGG-UGC-ACaGGC- -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 25779 | 0.68 | 0.332662 |
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Target: 5'- gGGGCCgGCGCCgGcGCCGCGgGUgCGg -3' miRNA: 3'- -UUCGGgUGCGGgUaUGGUGCaCAgGC- -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 26946 | 0.68 | 0.332662 |
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Target: 5'- -cGCCgC-CGuCCCGaccGCCACGUGUCCa -3' miRNA: 3'- uuCGG-GuGC-GGGUa--UGGUGCACAGGc -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 5989 | 0.68 | 0.332662 |
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Target: 5'- gAAGCCCAgGUCCc--CCACGUGgguuaugCCGu -3' miRNA: 3'- -UUCGGGUgCGGGuauGGUGCACa------GGC- -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 31713 | 0.69 | 0.324628 |
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Target: 5'- -uGCCCGCGCCCccGuuACG-GUCUGa -3' miRNA: 3'- uuCGGGUGCGGGuaUggUGCaCAGGC- -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 15332 | 0.69 | 0.301397 |
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Target: 5'- gAGGCUgACGCCCAgcugGCCuuGCGUGUUg- -3' miRNA: 3'- -UUCGGgUGCGGGUa---UGG--UGCACAGgc -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 28845 | 0.69 | 0.293944 |
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Target: 5'- gAGGCCCACGgCCGUGCCggucgguugACGgcugCCGa -3' miRNA: 3'- -UUCGGGUGCgGGUAUGG---------UGCaca-GGC- -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 11657 | 0.78 | 0.072475 |
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Target: 5'- cGGCCCAUGUCCGcgGCCACGcgGUCCa -3' miRNA: 3'- uUCGGGUGCGGGUa-UGGUGCa-CAGGc -5' |
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| 4388 | 3' | -58.3 | NC_001720.1 | + | 40743 | 1.07 | 0.000514 |
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Target: 5'- cAAGCCCACGCCCAUACCACGUGUCCGa -3' miRNA: 3'- -UUCGGGUGCGGGUAUGGUGCACAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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