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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
4398 | 3' | -44.2 | NC_001724.1 | + | 513 | 0.67 | 0.80106 |
Target: 5'- aGUGGuUUCUGUACAGGCGCggugccUGGUGUu -3' miRNA: 3'- -UACU-AAGAUGUGUUUGCGa-----ACCAUAu -5' |
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4398 | 3' | -44.2 | NC_001724.1 | + | 4510 | 0.67 | 0.78885 |
Target: 5'- cAUGcgUgCUACAUuuACGCUgGGUAUGu -3' miRNA: 3'- -UACuaA-GAUGUGuuUGCGAaCCAUAU- -5' |
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4398 | 3' | -44.2 | NC_001724.1 | + | 4431 | 1.06 | 0.002243 |
Target: 5'- cAUGAUUCUACACAAACGCUUGGUAUAg -3' miRNA: 3'- -UACUAAGAUGUGUUUGCGAACCAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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