Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 444 | 5' | -53.7 | AC_000013.1 | + | 43701 | 0.66 | 0.775252 |
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Target: 5'- uGGGUUguguauuuaagCGgggUGGAAAG-CGUGCGGUg -3' miRNA: 3'- uCCCAG-----------GCaa-GCCUUUCaGCACGUCG- -5' |
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| 444 | 5' | -53.7 | AC_000013.1 | + | 10718 | 0.68 | 0.61104 |
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Target: 5'- uGGcGUCCGcaggCGGGAcagguuugcacGGcCGUGCGGCu -3' miRNA: 3'- uCC-CAGGCaa--GCCUU-----------UCaGCACGUCG- -5' |
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| 444 | 5' | -53.7 | AC_000013.1 | + | 12708 | 0.69 | 0.599838 |
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Target: 5'- gAGGGUUCGacgUCgcgcgcuccgGGGAGGUCGUG-GGCg -3' miRNA: 3'- -UCCCAGGCa--AG----------CCUUUCAGCACgUCG- -5' |
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| 444 | 5' | -53.7 | AC_000013.1 | + | 44236 | 0.72 | 0.420181 |
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Target: 5'- -cGGUCCGggCGGA--GUCG-GUAGCg -3' miRNA: 3'- ucCCAGGCaaGCCUuuCAGCaCGUCG- -5' |
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| 444 | 5' | -53.7 | AC_000013.1 | + | 12979 | 0.75 | 0.269918 |
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Target: 5'- uGGGUCCGUUCGGGcugAGGUCccugaGCuGCa -3' miRNA: 3'- uCCCAGGCAAGCCU---UUCAGca---CGuCG- -5' |
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| 444 | 5' | -53.7 | AC_000013.1 | + | 773 | 1.13 | 0.000659 |
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Target: 5'- cAGGGUCCGUUCGGAAAGUCGUGCAGCa -3' miRNA: 3'- -UCCCAGGCAAGCCUUUCAGCACGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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