Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 445 | 5' | -59.6 | AC_000013.1 | + | 38360 | 0.66 | 0.424103 |
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Target: 5'- cAGGUGGCGCcacCUGGuGGCuGUUA-GGCg -3' miRNA: 3'- cUCCAUCGUGc--GACC-CCG-CAGUgCCG- -5' |
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| 445 | 5' | -59.6 | AC_000013.1 | + | 5447 | 0.66 | 0.414766 |
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Target: 5'- uGAGGUGGUACuuuggGUUGguGGGUaguugGUCugGGCg -3' miRNA: 3'- -CUCCAUCGUG-----CGAC--CCCG-----CAGugCCG- -5' |
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| 445 | 5' | -59.6 | AC_000013.1 | + | 12578 | 0.66 | 0.414766 |
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Target: 5'- aGAGGUGGCuucGCGCaGcaGCGagCGCGGCg -3' miRNA: 3'- -CUCCAUCG---UGCGaCccCGCa-GUGCCG- -5' |
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| 445 | 5' | -59.6 | AC_000013.1 | + | 18180 | 0.67 | 0.361493 |
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Target: 5'- -uGGUAGaa-GC-GGGGCGggaACGGCg -3' miRNA: 3'- cuCCAUCgugCGaCCCCGCag-UGCCG- -5' |
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| 445 | 5' | -59.6 | AC_000013.1 | + | 12283 | 0.69 | 0.269906 |
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Target: 5'- aGAGG--GUACGC-GGGGCG-CGcCGGCg -3' miRNA: 3'- -CUCCauCGUGCGaCCCCGCaGU-GCCG- -5' |
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| 445 | 5' | -59.6 | AC_000013.1 | + | 6517 | 0.7 | 0.243823 |
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Target: 5'- aGGGUcGGCACGUuuccuaUGGcGGUG-CGCGGCg -3' miRNA: 3'- cUCCA-UCGUGCG------ACC-CCGCaGUGCCG- -5' |
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| 445 | 5' | -59.6 | AC_000013.1 | + | 986 | 1.12 | 0.000177 |
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Target: 5'- cGAGGUAGCACGCUGGGGCGUCACGGCa -3' miRNA: 3'- -CUCCAUCGUGCGACCCCGCAGUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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