Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 449 | 5' | -56.5 | AC_000013.1 | + | 13311 | 0.66 | 0.605336 |
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Target: 5'- aCACGUGCaaCGCCa--CCACCAGcAACa -3' miRNA: 3'- cGUGCACGg-GCGGcagGGUGGUU-UUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 11957 | 0.66 | 0.594305 |
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Target: 5'- cGCAcCGUGCUggaaGCCauugacgCCCugCAAAACg -3' miRNA: 3'- -CGU-GCACGGg---CGGca-----GGGugGUUUUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 13474 | 0.66 | 0.583306 |
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Target: 5'- aGCGCGUGCUgCGCCGgguaCugCGGAGg -3' miRNA: 3'- -CGUGCACGG-GCGGCagg-GugGUUUUg -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 6684 | 0.67 | 0.52909 |
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Target: 5'- -gACGaugGCCaGCgGUCCCuGCCGGGACg -3' miRNA: 3'- cgUGCa--CGGgCGgCAGGG-UGGUUUUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 32720 | 0.67 | 0.518456 |
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Target: 5'- aGUACGcUGCCUuCgGUCCCGCCuucccguAGCa -3' miRNA: 3'- -CGUGC-ACGGGcGgCAGGGUGGuu-----UUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 29572 | 0.68 | 0.46669 |
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Target: 5'- aCACGUGCCuCGCCGcagUCGgCGAGGCg -3' miRNA: 3'- cGUGCACGG-GCGGCag-GGUgGUUUUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 10799 | 0.68 | 0.446738 |
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Target: 5'- cGCACG-GCCgUGCaaaccuGUCCCGCCugcGGACg -3' miRNA: 3'- -CGUGCaCGG-GCGg-----CAGGGUGGu--UUUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 17144 | 0.7 | 0.363299 |
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Target: 5'- cGUACGUGCCCGgcgugaccagaCCGUCaCCGggucCUGAAGCa -3' miRNA: 3'- -CGUGCACGGGC-----------GGCAG-GGU----GGUUUUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 14020 | 0.71 | 0.329843 |
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Target: 5'- aGCAUGguauUGCCCGCgugcUGUgCCACUAAAACc -3' miRNA: 3'- -CGUGC----ACGGGCG----GCAgGGUGGUUUUG- -5' |
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| 449 | 5' | -56.5 | AC_000013.1 | + | 2491 | 1.12 | 0.000371 |
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Target: 5'- gGCACGUGCCCGCCGUCCCACCAAAACa -3' miRNA: 3'- -CGUGCACGGGCGGCAGGGUGGUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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