Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 450 | 5' | -49.8 | AC_000013.1 | + | 14228 | 0.66 | 0.909673 |
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Target: 5'- aGUUCAGGuugggguGUACAACAACcgucCGGuGCGg- -3' miRNA: 3'- -CAAGUCU-------CAUGUUGUUGu---GCC-CGCau -5' |
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| 450 | 5' | -49.8 | AC_000013.1 | + | 13248 | 0.66 | 0.895942 |
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Target: 5'- cGUUCGGA--ACGcgAGCGCGGGCGc- -3' miRNA: 3'- -CAAGUCUcaUGUugUUGUGCCCGCau -5' |
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| 450 | 5' | -49.8 | AC_000013.1 | + | 23710 | 0.68 | 0.816897 |
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Target: 5'- -aUUAGuGUugAACGcCAUGGGCGUu -3' miRNA: 3'- caAGUCuCAugUUGUuGUGCCCGCAu -5' |
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| 450 | 5' | -49.8 | AC_000013.1 | + | 43808 | 0.69 | 0.786186 |
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Target: 5'- cUUUGGGGUGCGAagguUACGGGUGUGu -3' miRNA: 3'- cAAGUCUCAUGUUguu-GUGCCCGCAU- -5' |
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| 450 | 5' | -49.8 | AC_000013.1 | + | 766 | 0.7 | 0.724757 |
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Target: 5'- cGUUCGGAaagucGUGCAGCAAaaugcgcuccggcacCACGGGCa-- -3' miRNA: 3'- -CAAGUCU-----CAUGUUGUU---------------GUGCCCGcau -5' |
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| 450 | 5' | -49.8 | AC_000013.1 | + | 2608 | 1.07 | 0.003574 |
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Target: 5'- uGUUCAGAGUACAACAACACGGGCGUAc -3' miRNA: 3'- -CAAGUCUCAUGUUGUUGUGCCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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