Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
451 | 3' | -49 | AC_000013.1 | + | 13768 | 0.66 | 0.943485 |
Target: 5'- aCGCCCGG-CCGAGCAGaccgcguucggGAAGAgCUgCg -3' miRNA: 3'- -GUGGGCUaGGCUUGUU-----------UUUCU-GAgGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 14550 | 0.67 | 0.899906 |
Target: 5'- gGCCCG-UCCGGcACGAGcuuAAGGCgUCCg -3' miRNA: 3'- gUGGGCuAGGCU-UGUUU---UUCUG-AGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 14706 | 0.67 | 0.899906 |
Target: 5'- cCAUCUGGUCCGGAUAAAu-GGCgcCCUg -3' miRNA: 3'- -GUGGGCUAGGCUUGUUUuuCUGa-GGA- -5' |
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451 | 3' | -49 | AC_000013.1 | + | 11153 | 0.68 | 0.868354 |
Target: 5'- -uUCCGGUCCGAGCAGAGuGuuUUCUa -3' miRNA: 3'- guGGGCUAGGCUUGUUUUuCugAGGA- -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39958 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 40498 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 40363 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 40228 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 40093 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 38745 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39824 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39689 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39554 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39419 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39284 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39149 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 39014 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 38880 | 0.69 | 0.850897 |
Target: 5'- gCACCCGcgCCGGACuu--AGuCUCUg -3' miRNA: 3'- -GUGGGCuaGGCUUGuuuuUCuGAGGa -5' |
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451 | 3' | -49 | AC_000013.1 | + | 5163 | 0.93 | 0.041575 |
Target: 5'- aCACCCGAUCCGAACAAAAA-ACUCCUu -3' miRNA: 3'- -GUGGGCUAGGCUUGUUUUUcUGAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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