Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 451 | 5' | -56 | AC_000013.1 | + | 18750 | 0.66 | 0.659056 |
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Target: 5'- --cGGAGGCC-GCAGGggCGGgGuaguUCGCc -3' miRNA: 3'- aguCCUCUGGuUGUCCa-GCCgC----AGCG- -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 1632 | 0.67 | 0.592947 |
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Target: 5'- --uGGAGAa-GAC-GGUCGGUGUCGg -3' miRNA: 3'- aguCCUCUggUUGuCCAGCCGCAGCg -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 15943 | 0.67 | 0.592947 |
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Target: 5'- aUCGGaGGGACUGGCGGGUaaCGGgaUCGCc -3' miRNA: 3'- -AGUC-CUCUGGUUGUCCA--GCCgcAGCG- -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 5288 | 0.67 | 0.560213 |
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Target: 5'- aCGGGGGAUCGAucCAGG-CGGUGgaGCa -3' miRNA: 3'- aGUCCUCUGGUU--GUCCaGCCGCagCG- -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 14380 | 0.68 | 0.506909 |
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Target: 5'- -aGGGAGAauCCAGC-GG-CGGCGagGCg -3' miRNA: 3'- agUCCUCU--GGUUGuCCaGCCGCagCG- -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 4680 | 0.69 | 0.475975 |
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Target: 5'- gCAGGAGGCgaggacguuguaCAGCAGcUUGGCGUUGg -3' miRNA: 3'- aGUCCUCUG------------GUUGUCcAGCCGCAGCg -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 24108 | 0.73 | 0.249876 |
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Target: 5'- aCGGGAaACCAGaguGGUCGGCGUCc- -3' miRNA: 3'- aGUCCUcUGGUUgu-CCAGCCGCAGcg -5' |
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| 451 | 5' | -56 | AC_000013.1 | + | 5127 | 1.13 | 0.000403 |
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Target: 5'- cUCAGGAGACCAACAGGUCGGCGUCGCa -3' miRNA: 3'- -AGUCCUCUGGUUGUCCAGCCGCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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