Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
457 | 3' | -54.9 | AC_000013.1 | + | 10455 | 0.66 | 0.708113 |
Target: 5'- aGACAC--GCGGCCcggCUUCGUUGCCc -3' miRNA: 3'- cCUGUGgcUGUCGGa--GAAGCAGUGGc -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 40897 | 0.66 | 0.697274 |
Target: 5'- cGACACCGAUguuAGCUUCUUCuggUACgGa -3' miRNA: 3'- cCUGUGGCUG---UCGGAGAAGca-GUGgC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 19730 | 0.66 | 0.686371 |
Target: 5'- cGGGCACCGcgGgAGCCgaggaCGUCACgGg -3' miRNA: 3'- -CCUGUGGC--UgUCGGagaa-GCAGUGgC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 10082 | 0.67 | 0.664419 |
Target: 5'- cGGucGCACCGACAcGCCUgCggaaagacgUCG-CGCCGu -3' miRNA: 3'- -CC--UGUGGCUGU-CGGA-Ga--------AGCaGUGGC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 4219 | 0.67 | 0.664419 |
Target: 5'- gGGACAgCGACAaCCUCggUGaCACCu -3' miRNA: 3'- -CCUGUgGCUGUcGGAGaaGCaGUGGc -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 14497 | 0.67 | 0.642345 |
Target: 5'- cGGACggGCCGACGGCgCg-UUCGcuuUCGCCa -3' miRNA: 3'- -CCUG--UGGCUGUCG-GagAAGC---AGUGGc -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 7081 | 0.68 | 0.609188 |
Target: 5'- ---gGCCGACAGCCcgucaUCggCGUCAUCu -3' miRNA: 3'- ccugUGGCUGUCGG-----AGaaGCAGUGGc -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 13110 | 0.68 | 0.598163 |
Target: 5'- uGGuACACCccaACAGCgUCUuucUCGUCAgCCGa -3' miRNA: 3'- -CC-UGUGGc--UGUCGgAGA---AGCAGU-GGC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 13265 | 0.68 | 0.575115 |
Target: 5'- cGGGCGCCGGCGGggaaggaCCacacCGUCACCa -3' miRNA: 3'- -CCUGUGGCUGUC-------GGagaaGCAGUGGc -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 1723 | 0.69 | 0.532922 |
Target: 5'- cGuCGCCGAUgcgggccaAGCCUCUUCcGaCACCGa -3' miRNA: 3'- cCuGUGGCUG--------UCGGAGAAG-CaGUGGC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 13342 | 0.7 | 0.460338 |
Target: 5'- cGGCACCGACAGCagCUUCcgcaagcggCGCCGa -3' miRNA: 3'- cCUGUGGCUGUCGgaGAAGca-------GUGGC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 8742 | 0.72 | 0.357995 |
Target: 5'- uGGACACCacgcGCAuCCUCUUCGaacaggaccUCACCGa -3' miRNA: 3'- -CCUGUGGc---UGUcGGAGAAGC---------AGUGGC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 11844 | 0.73 | 0.309286 |
Target: 5'- aGACGCCGACguGGCg-C-UCGUCACCGa -3' miRNA: 3'- cCUGUGGCUG--UCGgaGaAGCAGUGGC- -5' |
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457 | 3' | -54.9 | AC_000013.1 | + | 8119 | 1.11 | 0.000692 |
Target: 5'- gGGACACCGACAGCCUCUUCGUCACCGa -3' miRNA: 3'- -CCUGUGGCUGUCGGAGAAGCAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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