Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 458 | 3' | -49.6 | AC_000013.1 | + | 25228 | 0.66 | 0.940553 |
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Target: 5'- cGGAA-UCgGAUUggaGCCAaccaaagacgaggccGCUGCAGGAAGc -3' miRNA: 3'- -CCUUcAG-CUAG---UGGU---------------UGACGUCCUUC- -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 34959 | 0.66 | 0.932848 |
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Target: 5'- uGGAGGUgcccauauaaaCGuucCGCCGACgGCAGGAu- -3' miRNA: 3'- -CCUUCA-----------GCua-GUGGUUGaCGUCCUuc -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 28315 | 0.66 | 0.926978 |
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Target: 5'- gGGAAG-CGAUCGaguCCGACgGCgaaacucuAGGAAGc -3' miRNA: 3'- -CCUUCaGCUAGU---GGUUGaCG--------UCCUUC- -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 31386 | 0.67 | 0.907527 |
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Target: 5'- aGGAAGUUaccaggccauugGAaUACCAugACUGUAGGAAa -3' miRNA: 3'- -CCUUCAG------------CUaGUGGU--UGACGUCCUUc -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 30192 | 0.67 | 0.877394 |
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Target: 5'- aGGAGUCGAuuUCGCCA--UGUcGGAGGc -3' miRNA: 3'- cCUUCAGCU--AGUGGUugACGuCCUUC- -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 44427 | 0.68 | 0.869143 |
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Target: 5'- gGGGAuUCGGUCACCGAUggggucgguUGCGGuGAGa -3' miRNA: 3'- -CCUUcAGCUAGUGGUUG---------ACGUCcUUC- -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 28330 | 0.74 | 0.546093 |
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Target: 5'- gGGAGcGUCGGUCucuuCCGACgGCGGGGGu -3' miRNA: 3'- -CCUU-CAGCUAGu---GGUUGaCGUCCUUc -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 29655 | 0.81 | 0.230522 |
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Target: 5'- aGGAGGUCuGGcgccUCGCCGACUGCGGcGAGGc -3' miRNA: 3'- -CCUUCAG-CU----AGUGGUUGACGUC-CUUC- -5' |
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| 458 | 3' | -49.6 | AC_000013.1 | + | 8430 | 1.12 | 0.002105 |
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Target: 5'- cGGAAGUCGAUCACCAACUGCAGGAAGc -3' miRNA: 3'- -CCUUCAGCUAGUGGUUGACGUCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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