Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
459 | 5' | -53.3 | AC_000013.1 | + | 40351 | 0.73 | 0.36726 |
Target: 5'- gGCCGGCAggGGGCGCC-CGUGCugggcgCCg -3' miRNA: 3'- gUGGUUGUa-CCUGUGGuGCGCGua----GG- -5' |
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459 | 5' | -53.3 | AC_000013.1 | + | 40486 | 0.73 | 0.36726 |
Target: 5'- gGCCGGCAggGGGCGCC-CGUGCugggcgCCg -3' miRNA: 3'- gUGGUUGUa-CCUGUGGuGCGCGua----GG- -5' |
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459 | 5' | -53.3 | AC_000013.1 | + | 41412 | 0.69 | 0.559841 |
Target: 5'- gCGCCAcCAUGGAgucaACUAC-CGUAUCCu -3' miRNA: 3'- -GUGGUuGUACCUg---UGGUGcGCGUAGG- -5' |
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459 | 5' | -53.3 | AC_000013.1 | + | 41459 | 0.66 | 0.790825 |
Target: 5'- gCGCuCGACuucaAUGGAUGCCGC-CGUAUCa -3' miRNA: 3'- -GUG-GUUG----UACCUGUGGUGcGCGUAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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