miRNA: 3'- guUGGACGCAGCCCa------GGACuCU-CUGG- -5'
miRNA: 3'- gUUGGaCGcaGCCCAGGa-CUCUC-UGG- -5'
miRNA: 3'- -GuuGGACGCAGCCCA----GGACU---CUcUGG- -5'
miRNA: 3'- gUUGGA---------CGCAGCCCaGGACUCUCUGg -5'
miRNA: 3'- -GUUGGACGCAGCCCAGGACUCUCUGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 464 | 5' | -56 | AC_000013.1 | + | 16373 | 0.67 | 0.569152 |
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Target: 5'- -cGCCUGUGUCGGGcgugcccCCUGcGAuGGCUc -3' miRNA: 3'- guUGGACGCAGCCCa------GGACuCU-CUGG- -5' |
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| 464 | 5' | -56 | AC_000013.1 | + | 43477 | 0.68 | 0.536752 |
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Target: 5'- gAGCCaGCucCGGGUCCcgGGGAGcGCCa -3' miRNA: 3'- gUUGGaCGcaGCCCAGGa-CUCUC-UGG- -5' |
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| 464 | 5' | -56 | AC_000013.1 | + | 20223 | 0.71 | 0.336145 |
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Target: 5'- uCggUCUGCGUCGGGUaaaaCCUGAucuGAaGCCu -3' miRNA: 3'- -GuuGGACGCAGCCCA----GGACU---CUcUGG- -5' |
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| 464 | 5' | -56 | AC_000013.1 | + | 16043 | 0.77 | 0.149191 |
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Target: 5'- aGACCUagcggauuaGCGUUGGG-CCUGAGGGGCa -3' miRNA: 3'- gUUGGA---------CGCAGCCCaGGACUCUCUGg -5' |
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| 464 | 5' | -56 | AC_000013.1 | + | 11353 | 1.13 | 0.000399 |
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Target: 5'- aCAACCUGCGUCGGGUCCUGAGAGACCg -3' miRNA: 3'- -GUUGGACGCAGCCCAGGACUCUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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