Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 467 | 3' | -58.1 | AC_000013.1 | + | 18540 | 0.66 | 0.489964 |
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Target: 5'- gGAGUCuUCGGCGGgaagCGGUugACGCccuCCg -3' miRNA: 3'- -UUCAGcAGCCGCCa---GUCG--UGCGcu-GG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 44469 | 0.66 | 0.479808 |
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Target: 5'- ----gGUCGGaCGGUUagaAGCGCGgGACUg -3' miRNA: 3'- uucagCAGCC-GCCAG---UCGUGCgCUGG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 2216 | 0.67 | 0.469756 |
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Target: 5'- aAAGUCGguuUCGGgGGUCAaCAUGUagucGGCCa -3' miRNA: 3'- -UUCAGC---AGCCgCCAGUcGUGCG----CUGG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 7778 | 0.67 | 0.440268 |
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Target: 5'- cAGUCGUaCGGCgcGGUCAGCGCcauuccCGAgCu -3' miRNA: 3'- uUCAGCA-GCCG--CCAGUCGUGc-----GCUgG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 8855 | 0.68 | 0.375805 |
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Target: 5'- -uGUCGUC-GUGGagGGC-CGUGACCg -3' miRNA: 3'- uuCAGCAGcCGCCagUCGuGCGCUGG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 19752 | 0.75 | 0.133339 |
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Target: 5'- aGGGUCGUCGGcCGGUCcguuucgGGCAcCGCGggaGCCg -3' miRNA: 3'- -UUCAGCAGCC-GCCAG-------UCGU-GCGC---UGG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 20026 | 0.78 | 0.090331 |
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Target: 5'- gGGGUCG-CGGUGGUCAGgucggguguaaACGCGGCCa -3' miRNA: 3'- -UUCAGCaGCCGCCAGUCg----------UGCGCUGG- -5' |
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| 467 | 3' | -58.1 | AC_000013.1 | + | 13742 | 1.08 | 0.000477 |
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Target: 5'- gAAGUCGUCGGCGGUCAGCACGCGACCc -3' miRNA: 3'- -UUCAGCAGCCGCCAGUCGUGCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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