Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 471 | 3' | -57.1 | AC_000013.1 | + | 18907 | 0.66 | 0.579391 |
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Target: 5'- --aUCAUcGCggCCGCUAUCGGGGCGAu- -3' miRNA: 3'- ccaAGUA-CG--GGUGGUGGCCCCGUUcc -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 15948 | 0.66 | 0.568564 |
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Target: 5'- --aUgAUGUCgCGCCugaacuuaCGGGGCGAGGg -3' miRNA: 3'- ccaAgUACGG-GUGGug------GCCCCGUUCC- -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 18760 | 0.66 | 0.557789 |
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Target: 5'- aGGgcg--GCCUcggagGCCGCaGGGGCGGGGu -3' miRNA: 3'- -CCaaguaCGGG-----UGGUGgCCCCGUUCC- -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 12280 | 0.67 | 0.515346 |
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Target: 5'- gGGUacgCGggGCgCGCCGgCGGuGGCGGGGa -3' miRNA: 3'- -CCAa--GUa-CGgGUGGUgGCC-CCGUUCC- -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 17989 | 0.67 | 0.484391 |
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Target: 5'- uGGggCAUGCgUCGCCGCUcgucuaugcguGGGGUcGGGa -3' miRNA: 3'- -CCaaGUACG-GGUGGUGG-----------CCCCGuUCC- -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 7477 | 0.69 | 0.406646 |
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Target: 5'- aGGUUCuucgugGCCCACCG--GGGGCAc-- -3' miRNA: 3'- -CCAAGua----CGGGUGGUggCCCCGUucc -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 27830 | 0.7 | 0.353734 |
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Target: 5'- aGGUaggCAuuUGCCCAUaguuCCGGGGUAAGu -3' miRNA: 3'- -CCAa--GU--ACGGGUGgu--GGCCCCGUUCc -5' |
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| 471 | 3' | -57.1 | AC_000013.1 | + | 17634 | 1.12 | 0.000343 |
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Target: 5'- aGGUUCAUGCCCACCACCGGGGCAAGGu -3' miRNA: 3'- -CCAAGUACGGGUGGUGGCCCCGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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