Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 473 | 3' | -54.2 | AC_000013.1 | + | 13257 | 0.66 | 0.684709 |
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Target: 5'- -cGCGAGC---GCgGGCGCCGGCGGg- -3' miRNA: 3'- uaUGUUCGacuCG-CCGUGGCUGCUau -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 18592 | 0.66 | 0.684709 |
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Target: 5'- cUACcguuGaCUGAGCGGCGCguuCGACGGg- -3' miRNA: 3'- uAUGuu--C-GACUCGCCGUG---GCUGCUau -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 13494 | 0.66 | 0.638915 |
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Target: 5'- uUGCGGGCUG-GCGGCgggaGCgGACGu-- -3' miRNA: 3'- uAUGUUCGACuCGCCG----UGgCUGCuau -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 7510 | 0.67 | 0.570228 |
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Target: 5'- --uCAGGCUGcucGCGGCGgCGGCGGc- -3' miRNA: 3'- uauGUUCGACu--CGCCGUgGCUGCUau -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 28296 | 0.68 | 0.51439 |
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Target: 5'- -gACGGGCUGuacGCGGCACgGgaaGCGAUc -3' miRNA: 3'- uaUGUUCGACu--CGCCGUGgC---UGCUAu -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 13349 | 0.69 | 0.481965 |
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Target: 5'- -gACAgcAGCUuccgcaAGCGGCGCCGACGcgAa -3' miRNA: 3'- uaUGU--UCGAc-----UCGCCGUGGCUGCuaU- -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 7975 | 0.7 | 0.400847 |
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Target: 5'- aGUGCAAGCgGuGCGGCGgCGACa--- -3' miRNA: 3'- -UAUGUUCGaCuCGCCGUgGCUGcuau -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 23847 | 0.73 | 0.281608 |
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Target: 5'- -cGCGAGC-GAGCGGUAUCGgaaGCGAUAu -3' miRNA: 3'- uaUGUUCGaCUCGCCGUGGC---UGCUAU- -5' |
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| 473 | 3' | -54.2 | AC_000013.1 | + | 23942 | 1.05 | 0.001531 |
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Target: 5'- cAUACAAGCUGAGCGGCACCGACGAUAu -3' miRNA: 3'- -UAUGUUCGACUCGCCGUGGCUGCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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