Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 475 | 5' | -52.4 | AC_000013.1 | + | 12806 | 0.68 | 0.711239 |
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Target: 5'- cGGUGAGGCACGUccagaaaagccUCCUGGgucGCugCGc -3' miRNA: 3'- -CCGCUCCGUGCAc----------AGGAUUa--UGugGU- -5' |
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| 475 | 5' | -52.4 | AC_000013.1 | + | 16063 | 0.68 | 0.688901 |
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Target: 5'- gGGCcugaGGGGCACGgGUCCUGcgcGCGCUu -3' miRNA: 3'- -CCG----CUCCGUGCaCAGGAUua-UGUGGu -5' |
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| 475 | 5' | -52.4 | AC_000013.1 | + | 2497 | 0.7 | 0.598157 |
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Target: 5'- cGGUGcGGCACGUGcCCgccguccCACCAa -3' miRNA: 3'- -CCGCuCCGUGCACaGGauuau--GUGGU- -5' |
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| 475 | 5' | -52.4 | AC_000013.1 | + | 2433 | 0.72 | 0.477311 |
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Target: 5'- cGGCG-GGCACGUG-CC----GCACCGc -3' miRNA: 3'- -CCGCuCCGUGCACaGGauuaUGUGGU- -5' |
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| 475 | 5' | -52.4 | AC_000013.1 | + | 38611 | 0.73 | 0.436321 |
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Target: 5'- aGGCGGGGCAUaaGcCCUAG-GCACCAc -3' miRNA: 3'- -CCGCUCCGUGcaCaGGAUUaUGUGGU- -5' |
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| 475 | 5' | -52.4 | AC_000013.1 | + | 136 | 0.76 | 0.285918 |
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Target: 5'- gGGCGAGGCACGgagCCgacuuucugGCACCGa -3' miRNA: 3'- -CCGCUCCGUGCacaGGauua-----UGUGGU- -5' |
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| 475 | 5' | -52.4 | AC_000013.1 | + | 29630 | 1.11 | 0.001075 |
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Target: 5'- cGGCGAGGCACGUGUCCUAAUACACCAc -3' miRNA: 3'- -CCGCUCCGUGCACAGGAUUAUGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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