Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 478 | 3' | -53.6 | AC_000013.1 | + | 20390 | 0.66 | 0.720416 |
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Target: 5'- aGGCuucgcGGGGcGCGAGGggauuguauGCGGUUCCUCc -3' miRNA: 3'- aCUGu----UUUCuCGCUCU---------CGCCGAGGAG- -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 16421 | 0.66 | 0.709294 |
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Target: 5'- aGGCGAAGGAG-GAgGAGCGGCcggCaUCg -3' miRNA: 3'- aCUGUUUUCUCgCU-CUCGCCGa--GgAG- -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 41253 | 0.66 | 0.69809 |
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Target: 5'- aGAU-AAGGAGCGGuauuuaaGGCGGCUUCUUu -3' miRNA: 3'- aCUGuUUUCUCGCUc------UCGCCGAGGAG- -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 1341 | 0.67 | 0.664113 |
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Target: 5'- uUGACc-GAGAGCGAG-GCGGCggaCg- -3' miRNA: 3'- -ACUGuuUUCUCGCUCuCGCCGag-Gag -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 39079 | 0.69 | 0.539298 |
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Target: 5'- aGACAAGuccGGcGCGGGugcaguGGCGGC-CCUCg -3' miRNA: 3'- aCUGUUU---UCuCGCUC------UCGCCGaGGAG- -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 30322 | 0.69 | 0.506382 |
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Target: 5'- gGGCGGAGGAG-GAGgaAGCGGCggaaaccCCUCc -3' miRNA: 3'- aCUGUUUUCUCgCUC--UCGCCGa------GGAG- -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 28312 | 0.73 | 0.307547 |
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Target: 5'- cGAC-----GGCGGGGGUGGCUCUUCg -3' miRNA: 3'- aCUGuuuucUCGCUCUCGCCGAGGAG- -5' |
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| 478 | 3' | -53.6 | AC_000013.1 | + | 3860 | 1.11 | 0.000756 |
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Target: 5'- cUGACAAAAGAGCGAGAGCGGCUCCUCg -3' miRNA: 3'- -ACUGUUUUCUCGCUCUCGCCGAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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