Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 478 | 5' | -53.4 | AC_000013.1 | + | 10022 | 0.66 | 0.77396 |
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Target: 5'- -cGCGaggAGUcgUCGCG-CCCAgcgaCUCCCg -3' miRNA: 3'- uaUGCa--UCG--AGCGCuGGGUaa--GAGGG- -5' |
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| 478 | 5' | -53.4 | AC_000013.1 | + | 10464 | 0.66 | 0.742239 |
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Target: 5'- cUAC-UGGCUagacaCGCGGCCCGgcUUCguugCCCg -3' miRNA: 3'- uAUGcAUCGA-----GCGCUGGGU--AAGa---GGG- -5' |
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| 478 | 5' | -53.4 | AC_000013.1 | + | 22512 | 0.68 | 0.653368 |
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Target: 5'- gAUACGgccaaUUCGCGGgCCAcgaUUCUCCCu -3' miRNA: 3'- -UAUGCauc--GAGCGCUgGGU---AAGAGGG- -5' |
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| 478 | 5' | -53.4 | AC_000013.1 | + | 16922 | 0.68 | 0.630705 |
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Target: 5'- -aGCGUagGGCUCGgGGCUCGUgggagCUgCCg -3' miRNA: 3'- uaUGCA--UCGAGCgCUGGGUAa----GAgGG- -5' |
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| 478 | 5' | -53.4 | AC_000013.1 | + | 19867 | 0.74 | 0.318321 |
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Target: 5'- cAUGCGacggcGGCUaacUGCGACCCcgUUUCCCg -3' miRNA: 3'- -UAUGCa----UCGA---GCGCUGGGuaAGAGGG- -5' |
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| 478 | 5' | -53.4 | AC_000013.1 | + | 3894 | 1.11 | 0.000857 |
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Target: 5'- cAUACGUAGCUCGCGACCCAUUCUCCCa -3' miRNA: 3'- -UAUGCAUCGAGCGCUGGGUAAGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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