Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 481 | 3' | -54 | AC_000013.1 | + | 16473 | 0.66 | 0.69809 |
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Target: 5'- gCACCGUCGCCggguAGCGUUgAGgAGCcaucGCAg -3' miRNA: 3'- -GUGGCAGUGG----UCGUAG-UCgUCGu---UGU- -5' |
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| 481 | 3' | -54 | AC_000013.1 | + | 27978 | 0.66 | 0.675487 |
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Target: 5'- aACCGgggaaaCGCUAGCcacuUCGGCGGCGAg- -3' miRNA: 3'- gUGGCa-----GUGGUCGu---AGUCGUCGUUgu -5' |
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| 481 | 3' | -54 | AC_000013.1 | + | 7072 | 0.68 | 0.584231 |
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Target: 5'- -cCCGUCAUCGGCGUCAucuaCGGCccGACGg -3' miRNA: 3'- guGGCAGUGGUCGUAGUc---GUCG--UUGU- -5' |
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| 481 | 3' | -54 | AC_000013.1 | + | 43559 | 0.68 | 0.57291 |
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Target: 5'- uGCgGgaACCAGC--CAGCAGCAGCAg -3' miRNA: 3'- gUGgCagUGGUCGuaGUCGUCGUUGU- -5' |
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| 481 | 3' | -54 | AC_000013.1 | + | 13320 | 0.69 | 0.517264 |
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Target: 5'- aCGCCacCACCAGCAaCAGCAaCGGCAc -3' miRNA: 3'- -GUGGcaGUGGUCGUaGUCGUcGUUGU- -5' |
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| 481 | 3' | -54 | AC_000013.1 | + | 6927 | 0.71 | 0.375801 |
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Target: 5'- aACCGUUuCCGGgAUCGGUuGCAGCAu -3' miRNA: 3'- gUGGCAGuGGUCgUAGUCGuCGUUGU- -5' |
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| 481 | 3' | -54 | AC_000013.1 | + | 13287 | 1.09 | 0.000911 |
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Target: 5'- aCACCGUCACCAGCAUCAGCAGCAACAc -3' miRNA: 3'- -GUGGCAGUGGUCGUAGUCGUCGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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