Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 483 | 3' | -53.3 | AC_000013.1 | + | 16890 | 0.66 | 0.769022 |
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Target: 5'- ----uGCCUgGAGGCaccGGAUCGCGGUAa -3' miRNA: 3'- caaguUGGGgCUCCG---CUUAGUGUCGU- -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 1172 | 0.66 | 0.758449 |
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Target: 5'- uUUCAACgUCCGucgGGGCGAucgCAUAGCu -3' miRNA: 3'- cAAGUUG-GGGC---UCCGCUua-GUGUCGu -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 37607 | 0.68 | 0.635767 |
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Target: 5'- -aUUGAaUuuGAGGCaGAAUCACAGCAu -3' miRNA: 3'- caAGUUgGggCUCCG-CUUAGUGUCGU- -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 1026 | 0.7 | 0.520986 |
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Target: 5'- uUUCAAuucCCCCGAGGCGuguacgggcgCAUAGCc -3' miRNA: 3'- cAAGUU---GGGGCUCCGCuua-------GUGUCGu -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 13724 | 0.7 | 0.512266 |
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Target: 5'- cGUUC-ACgCCGGGuGCGAAUCACuuGCGc -3' miRNA: 3'- -CAAGuUGgGGCUC-CGCUUAGUGu-CGU- -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 994 | 0.7 | 0.509012 |
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Target: 5'- --gCAGCCCCcgagguagcacgcuGGGGCG--UCACGGCAa -3' miRNA: 3'- caaGUUGGGG--------------CUCCGCuuAGUGUCGU- -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 23767 | 0.71 | 0.43789 |
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Target: 5'- --cCGugCCCGAGggagauuGCGGAUUGCAGCAc -3' miRNA: 3'- caaGUugGGGCUC-------CGCUUAGUGUCGU- -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 7889 | 0.73 | 0.328504 |
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Target: 5'- -cUCGACCCCGAGuGCG-GUCACgugggaAGCGg -3' miRNA: 3'- caAGUUGGGGCUC-CGCuUAGUG------UCGU- -5' |
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| 483 | 3' | -53.3 | AC_000013.1 | + | 16616 | 1.09 | 0.001064 |
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Target: 5'- cGUUCAACCCCGAGGCGAAUCACAGCAa -3' miRNA: 3'- -CAAGUUGGGGCUCCGCUUAGUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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