Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 486 | 5' | -52.9 | AC_000013.1 | + | 7453 | 0.67 | 0.736898 |
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Target: 5'- -cGCG-AgCAGCCUGAGGggGGcuAGAg -3' miRNA: 3'- caUGCaUgGUCGGGCUUCuuCC--UCUg -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 28354 | 0.67 | 0.703754 |
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Target: 5'- -cGCGUA-CAGCCCGucuguauGGAAGGGaGCg -3' miRNA: 3'- caUGCAUgGUCGGGCu------UCUUCCUcUG- -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 1128 | 0.67 | 0.692539 |
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Target: 5'- uUACG-GCCaAGCCCGAAcGAGG-GGCu -3' miRNA: 3'- cAUGCaUGG-UCGGGCUUcUUCCuCUG- -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 13566 | 0.68 | 0.681261 |
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Target: 5'- cUACGUccgcucccgccGCCAGCCCGcAAacGGGAGGa -3' miRNA: 3'- cAUGCA-----------UGGUCGGGC-UUcuUCCUCUg -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 12270 | 0.68 | 0.669933 |
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Target: 5'- -gGCGcGCCGGCgguggCGggGAcGGAGGCg -3' miRNA: 3'- caUGCaUGGUCGg----GCuuCUuCCUCUG- -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 11145 | 0.69 | 0.59021 |
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Target: 5'- -gGCGUGucuuCCGGUCCGAgcAGAguguuuucuagGGGAGGCg -3' miRNA: 3'- caUGCAU----GGUCGGGCU--UCU-----------UCCUCUG- -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 19713 | 0.7 | 0.512266 |
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Target: 5'- aUGCGU-CCuccGCCCGcgaaGAGGAGGAGAa -3' miRNA: 3'- cAUGCAuGGu--CGGGC----UUCUUCCUCUg -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 28374 | 0.71 | 0.459269 |
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Target: 5'- ---aGUACCAuCUCGGAGGAGGAGGa -3' miRNA: 3'- caugCAUGGUcGGGCUUCUUCCUCUg -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 18206 | 0.72 | 0.409331 |
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Target: 5'- gGUACGUACCAGCauuacgCGAguagugguAGAAGcGGGGCg -3' miRNA: 3'- -CAUGCAUGGUCGg-----GCU--------UCUUC-CUCUG- -5' |
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| 486 | 5' | -52.9 | AC_000013.1 | + | 18153 | 1.1 | 0.001031 |
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Target: 5'- gGUACGUACCAGCCCGAAGAAGGAGACg -3' miRNA: 3'- -CAUGCAUGGUCGGGCUUCUUCCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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