Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 489 | 3' | -56.1 | AC_000013.1 | + | 12660 | 0.66 | 0.607571 |
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Target: 5'- -aCGGGGAcgccGCGCUcgcugcugcgcgaAGCCACcucUCGGg -3' miRNA: 3'- ugGCCCCUu---UGCGA-------------UCGGUGa--AGCCg -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 25070 | 0.67 | 0.564611 |
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Target: 5'- cAUCGaGGGAGGCuGCaggAGCCcguACcUCGGCg -3' miRNA: 3'- -UGGC-CCCUUUG-CGa--UCGG---UGaAGCCG- -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 132 | 0.68 | 0.510822 |
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Target: 5'- uGCCGGGcGAGGCaCggAGCCgACUUucUGGCa -3' miRNA: 3'- -UGGCCC-CUUUGcGa-UCGG-UGAA--GCCG- -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 18647 | 0.68 | 0.510822 |
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Target: 5'- gGCCGuaguGGGcGACGgUaccgccacAGCCACgUUCGGCg -3' miRNA: 3'- -UGGC----CCCuUUGCgA--------UCGGUG-AAGCCG- -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 20009 | 0.69 | 0.439451 |
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Target: 5'- gUCGGGuGuAAACGC-GGCCAUagCGGCa -3' miRNA: 3'- uGGCCC-C-UUUGCGaUCGGUGaaGCCG- -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 19678 | 0.73 | 0.264413 |
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Target: 5'- cUCGGGGAuggGGCGUguugucggucgAGgCACUUCGGCg -3' miRNA: 3'- uGGCCCCU---UUGCGa----------UCgGUGAAGCCG- -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 19391 | 0.76 | 0.149088 |
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Target: 5'- gGCCGGGGAAACcCUAGgCGCUcuuaccgacauaggUCGGUu -3' miRNA: 3'- -UGGCCCCUUUGcGAUCgGUGA--------------AGCCG- -5' |
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| 489 | 3' | -56.1 | AC_000013.1 | + | 27978 | 1.12 | 0.000401 |
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Target: 5'- aACCGGGGAAACGCUAGCCACUUCGGCg -3' miRNA: 3'- -UGGCCCCUUUGCGAUCGGUGAAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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