Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
491 | 3' | -55.8 | AC_000013.1 | + | 28039 | 0.73 | 0.27968 |
Target: 5'- aGGGGGuGCGUCUUuac--CGuCCGCUCCc -3' miRNA: 3'- -CCCCC-CGUAGAGcuuuuGC-GGCGAGG- -5' |
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491 | 3' | -55.8 | AC_000013.1 | + | 9452 | 0.77 | 0.143712 |
Target: 5'- aGGGGGGCG-CUCGAccgugcuCGCgCGUUCCu -3' miRNA: 3'- -CCCCCCGUaGAGCUuuu----GCG-GCGAGG- -5' |
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491 | 3' | -55.8 | AC_000013.1 | + | 28443 | 1.15 | 0.000305 |
Target: 5'- aGGGGGGCAUCUCGAAAACGCCGCUCCg -3' miRNA: 3'- -CCCCCCGUAGAGCUUUUGCGGCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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