Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 492 | 5' | -54.3 | AC_000013.1 | + | 14481 | 0.66 | 0.715062 |
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Target: 5'- cGCCUUaAgCUCGUGccgGACGG-GCCGa- -3' miRNA: 3'- -CGGAGaUgGAGCAU---UUGCCgCGGCau -5' |
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| 492 | 5' | -54.3 | AC_000013.1 | + | 15399 | 0.67 | 0.648232 |
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Target: 5'- uCCUgCU-CUUCGcucaauucGAGCGGCGCCGUAu -3' miRNA: 3'- cGGA-GAuGGAGCa-------UUUGCCGCGGCAU- -5' |
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| 492 | 5' | -54.3 | AC_000013.1 | + | 22921 | 0.68 | 0.591994 |
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Target: 5'- uGCCUCgGCCaUCGUGAAUaccgggucuaGaGCGCCGg- -3' miRNA: 3'- -CGGAGaUGG-AGCAUUUG----------C-CGCGGCau -5' |
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| 492 | 5' | -54.3 | AC_000013.1 | + | 9367 | 0.68 | 0.569678 |
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Target: 5'- cGCgCUCgACgUCGUGuacguCGGCGUCGUGa -3' miRNA: 3'- -CG-GAGaUGgAGCAUuu---GCCGCGGCAU- -5' |
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| 492 | 5' | -54.3 | AC_000013.1 | + | 1462 | 0.74 | 0.278464 |
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Target: 5'- aGCCucguuaUCUGCCUCGaGAGCGGcCGCCa-- -3' miRNA: 3'- -CGG------AGAUGGAGCaUUUGCC-GCGGcau -5' |
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| 492 | 5' | -54.3 | AC_000013.1 | + | 12660 | 0.78 | 0.157666 |
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Target: 5'- cCCUCUGCCUCGcagaUGAACGuGCGCCa-- -3' miRNA: 3'- cGGAGAUGGAGC----AUUUGC-CGCGGcau -5' |
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| 492 | 5' | -54.3 | AC_000013.1 | + | 28822 | 1.09 | 0.000885 |
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Target: 5'- cGCCUCUACCUCGUAAACGGCGCCGUAg -3' miRNA: 3'- -CGGAGAUGGAGCAUUUGCCGCGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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