Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 493 | 5' | -62.4 | AC_000013.1 | + | 4640 | 0.66 | 0.33752 |
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Target: 5'- gCCCUucgucgacaGAGCCCCGUC--CACGCucGCGc -3' miRNA: 3'- -GGGA---------CUCGGGGCGGcaGUGCGcuCGC- -5' |
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| 493 | 5' | -62.4 | AC_000013.1 | + | 16177 | 0.66 | 0.329772 |
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Target: 5'- aUCCgGAGCCUgacaUGCCGcCgACGCGAGgCGu -3' miRNA: 3'- -GGGaCUCGGG----GCGGCaG-UGCGCUC-GC- -5' |
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| 493 | 5' | -62.4 | AC_000013.1 | + | 41562 | 0.67 | 0.286056 |
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Target: 5'- uCCCgUGAGCgCCGUgaaGUUAUGCagGAGCGg -3' miRNA: 3'- -GGG-ACUCGgGGCGg--CAGUGCG--CUCGC- -5' |
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| 493 | 5' | -62.4 | AC_000013.1 | + | 3606 | 0.69 | 0.207204 |
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Target: 5'- uCCCUGuGCgaCCGCgGUaACGCGAuGCGa -3' miRNA: 3'- -GGGACuCGg-GGCGgCAgUGCGCU-CGC- -5' |
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| 493 | 5' | -62.4 | AC_000013.1 | + | 7434 | 0.71 | 0.160003 |
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Target: 5'- uCCgUGcGGCCgCCGCCG-C-CGCGAGCa -3' miRNA: 3'- -GGgAC-UCGG-GGCGGCaGuGCGCUCGc -5' |
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| 493 | 5' | -62.4 | AC_000013.1 | + | 29315 | 1.1 | 0.000152 |
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Target: 5'- aCCCUGAGCCCCGCCGUCACGCGAGCGa -3' miRNA: 3'- -GGGACUCGGGGCGGCAGUGCGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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