Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 503 | 5' | -55.4 | AC_000013.1 | + | 6329 | 0.67 | 0.56252 |
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Target: 5'- aGUCGGAGaCGGGGUCgGGGGAguaagugagcCAGCu -3' miRNA: 3'- gCGGUUUC-GUCUCAGgCUCCU----------GUCGu -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 20002 | 0.67 | 0.555896 |
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Target: 5'- uCGCCGguccGAGCAcccgggaauaccuGUCCGAGGACcuucAGCAg -3' miRNA: 3'- -GCGGU----UUCGUcu-----------CAGGCUCCUG----UCGU- -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 23132 | 0.68 | 0.518839 |
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Target: 5'- cCGUCGAAGCGGuucaauGUCCGuguucGGCGGCGu -3' miRNA: 3'- -GCGGUUUCGUCu-----CAGGCuc---CUGUCGU- -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 14395 | 0.69 | 0.456073 |
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Target: 5'- gCGgCGAGGCGGAG---GAGGACGGCu -3' miRNA: 3'- -GCgGUUUCGUCUCaggCUCCUGUCGu -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 19335 | 0.71 | 0.361432 |
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Target: 5'- gGCCAgaucAAGCAGGGcUUUaAGGACAGCGg -3' miRNA: 3'- gCGGU----UUCGUCUC-AGGcUCCUGUCGU- -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 44959 | 0.73 | 0.246885 |
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Target: 5'- gGCgGGAGCGGuAGUgCGAGGugACAGCAg -3' miRNA: 3'- gCGgUUUCGUC-UCAgGCUCC--UGUCGU- -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 105 | 0.73 | 0.246885 |
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Target: 5'- gGCgGGAGCGGuAGUgCGAGGugACAGCAg -3' miRNA: 3'- gCGgUUUCGUC-UCAgGCUCC--UGUCGU- -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 13494 | 0.74 | 0.227935 |
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Target: 5'- cCGCgGAAGCGGA---CGAGGGCAGCGc -3' miRNA: 3'- -GCGgUUUCGUCUcagGCUCCUGUCGU- -5' |
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| 503 | 5' | -55.4 | AC_000013.1 | + | 43500 | 1.09 | 0.000686 |
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Target: 5'- gCGCCAAAGCAGAGUCCGAGGACAGCAg -3' miRNA: 3'- -GCGGUUUCGUCUCAGGCUCCUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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