Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5031 | 3' | -59.5 | NC_001734.1 | + | 23256 | 0.67 | 0.28938 |
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Target: 5'- aAGCCcCCUUCAguugaacccACUCCCUGC-GUCAu -3' miRNA: 3'- -UCGGcGGAGGUa--------UGAGGGGCGuCAGU- -5' |
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| 5031 | 3' | -59.5 | NC_001734.1 | + | 10686 | 0.68 | 0.218813 |
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Target: 5'- -aCCGCCUucuauuuccgauagaCCGUuggaagucaagGCUUCCCGCAGUCu -3' miRNA: 3'- ucGGCGGA---------------GGUA-----------UGAGGGGCGUCAGu -5' |
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| 5031 | 3' | -59.5 | NC_001734.1 | + | 9877 | 0.69 | 0.199507 |
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Target: 5'- cGCCGCCUCU-UGCUUcauuugcaCCCGCGGgugCAc -3' miRNA: 3'- uCGGCGGAGGuAUGAG--------GGGCGUCa--GU- -5' |
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| 5031 | 3' | -59.5 | NC_001734.1 | + | 16636 | 0.69 | 0.188857 |
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Target: 5'- uGCCaGCCUCU--GCUCCUuacaggCGCGGUCAc -3' miRNA: 3'- uCGG-CGGAGGuaUGAGGG------GCGUCAGU- -5' |
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| 5031 | 3' | -59.5 | NC_001734.1 | + | 19660 | 0.71 | 0.14282 |
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Target: 5'- uGCaCGCCUCCAgucuCUCgaCCCGCGGUg- -3' miRNA: 3'- uCG-GCGGAGGUau--GAG--GGGCGUCAgu -5' |
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| 5031 | 3' | -59.5 | NC_001734.1 | + | 16537 | 1.08 | 0.000189 |
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Target: 5'- cAGCCGCCUCCAUACUCCCCGCAGUCAu -3' miRNA: 3'- -UCGGCGGAGGUAUGAGGGGCGUCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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