Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5058 | 5' | -52.5 | NC_001748.1 | + | 3587 | 0.66 | 0.248253 |
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Target: 5'- cUGGCUUCaauggUAgCCCAAAguAGGGUg -3' miRNA: 3'- -ACCGAAGgcuuaGUgGGGUUU--UCCCG- -5' |
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| 5058 | 5' | -52.5 | NC_001748.1 | + | 4027 | 0.66 | 0.233206 |
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Target: 5'- aGGuCUUCCgGggUCAUCUCAcaguuaauccuGAuguGGGCa -3' miRNA: 3'- aCC-GAAGG-CuuAGUGGGGU-----------UUu--CCCG- -5' |
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| 5058 | 5' | -52.5 | NC_001748.1 | + | 6377 | 0.67 | 0.212081 |
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Target: 5'- cGGCgaCCuGAUUAgUCCCGAAGGGGg -3' miRNA: 3'- aCCGaaGGcUUAGU-GGGGUUUUCCCg -5' |
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| 5058 | 5' | -52.5 | NC_001748.1 | + | 6346 | 0.67 | 0.212081 |
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Target: 5'- aUGGCcgCCGGAUucucCACCCCGucGAAGaagucgaacgcGGCa -3' miRNA: 3'- -ACCGaaGGCUUA----GUGGGGU--UUUC-----------CCG- -5' |
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| 5058 | 5' | -52.5 | NC_001748.1 | + | 2350 | 0.69 | 0.140544 |
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Target: 5'- aGGCUugcacuugggcaugcUUCGAAUUuCCUCAAuGGGGCu -3' miRNA: 3'- aCCGA---------------AGGCUUAGuGGGGUUuUCCCG- -5' |
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| 5058 | 5' | -52.5 | NC_001748.1 | + | 4468 | 1.12 | 0.000045 |
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Target: 5'- gUGGCUUCCGAAUCACCCCAAAAGGGCu -3' miRNA: 3'- -ACCGAAGGCUUAGUGGGGUUUUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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