Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5079 | 3' | -54.3 | NC_001786.1 | + | 5235 | 0.66 | 0.315064 |
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Target: 5'- gCGGUGguuuaGGAGGCGGUgugguucugagcacGGGUGCUc -3' miRNA: 3'- aGCCACagug-UCUUCGUCA--------------UCCGCGG- -5' |
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| 5079 | 3' | -54.3 | NC_001786.1 | + | 6451 | 0.66 | 0.308956 |
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Target: 5'- aCGGUGaauucCGCAGAGGUAGgcaguGGCuaguggcucuGCCg -3' miRNA: 3'- aGCCACa----GUGUCUUCGUCau---CCG----------CGG- -5' |
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| 5079 | 3' | -54.3 | NC_001786.1 | + | 562 | 0.67 | 0.2603 |
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Target: 5'- cUUGGUGgUACAGc-GUGGUAGGUGCg -3' miRNA: 3'- -AGCCACaGUGUCuuCGUCAUCCGCGg -5' |
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| 5079 | 3' | -54.3 | NC_001786.1 | + | 9951 | 0.74 | 0.076438 |
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Target: 5'- cUCGGUGuUCACAGgcGUguacccguuuauugGGgAGGCGCCu -3' miRNA: 3'- -AGCCAC-AGUGUCuuCG--------------UCaUCCGCGG- -5' |
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| 5079 | 3' | -54.3 | NC_001786.1 | + | 10024 | 1.12 | 0.000072 |
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Target: 5'- uUCGGUGUCACAGAAGCAGUAGGCGCCu -3' miRNA: 3'- -AGCCACAGUGUCUUCGUCAUCCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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