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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5102 | 5' | -53.5 | NC_001798.1 | + | 154239 | 1.08 | 0.004949 |
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Target: 5'- cCAGUCCGUGCUUGCCUAGCGAACUCAc -3' miRNA: 3'- -GUCAGGCACGAACGGAUCGCUUGAGU- -5' |
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| 5102 | 5' | -53.5 | NC_001798.1 | + | 136594 | 0.69 | 0.870984 |
|
Target: 5'- aCAGUCCGUGCgggggacggGCCcgGGCaacugcaacgcaGAGCUCu -3' miRNA: 3'- -GUCAGGCACGaa-------CGGa-UCG------------CUUGAGu -5' |
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| 5102 | 5' | -53.5 | NC_001798.1 | + | 128457 | 0.67 | 0.93441 |
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Target: 5'- gGGUCCGUGCUUcucGuCCcAGUG-GCUCAc -3' miRNA: 3'- gUCAGGCACGAA---C-GGaUCGCuUGAGU- -5' |
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| 5102 | 5' | -53.5 | NC_001798.1 | + | 52867 | 0.68 | 0.896631 |
|
Target: 5'- gAGUCCGUGCUgggGCCgcggguccgcgcGUGGACaUCAu -3' miRNA: 3'- gUCAGGCACGAa--CGGau----------CGCUUG-AGU- -5' |
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| 5102 | 5' | -53.5 | NC_001798.1 | + | 27824 | 0.67 | 0.929184 |
|
Target: 5'- gCGGUUacaCGUGCaugcgUGCCgAGUGAACUCu -3' miRNA: 3'- -GUCAG---GCACGa----ACGGaUCGCUUGAGu -5' |
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| 5102 | 5' | -53.5 | NC_001798.1 | + | 4091 | 0.71 | 0.794821 |
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Target: 5'- gGGUCCGggccggggcggGCUcgGCCcugGGCGGGCUCGg -3' miRNA: 3'- gUCAGGCa----------CGAa-CGGa--UCGCUUGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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